Membranes: Cell Membrane and Purple Dark

Cell Membranes and impartation Hands-On Labs, Inc. Version 42-0033-00-01 Lab Report Assistant This document is not meant to be a supervene upon for a formal science laboratory report. The Lab Report Assistant is simply a summary of the try outs questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to despatch students writing of lab reports by providing this information in an editable file which can be sent to an instructor. operate 1 Diffusion ObservationsData Table 1 Rate of diffusion in different temperatures ? C proceeding Temperature InitialTemp. InitialColor 5 10 15 20 25 30 60 Cold 3 vacuous white white white white white white Mostly white some easy Ambient 25 white twineedish Light blue purple blue purple blue purple purple purple smutty purple Hot 96 white purple DARKpurpleple Dark purple Dark purple Dark purple Dark purple Dark purple Questions A. issue a one-paragraph analysis of the results for the pro cedure. Provide reasons for the difference in the rate of diffusion at the different temperatures.The results of this lab were greatly influenced by the temperature of the KI solution. The bag placed in the heated solution began to change color (from white to light purple) almost immediately and changed to dark purple within 7minutes. The second bag in room temp. solution took about 5 minutes to see a slight change in color (from white to light blue) and about 20 to turn dark purple. The third bag in the cold temp. solution barely changed color at all at the end of the hour (bluish tint. ) concord to the text (p. 68), Beca hire the driving force for diffusion is the kinetic energy of the olecules themselves. The speed of diffusion is influenced by molecular size ( the smaller, the swift) and by temperature ( the warmer, the faster). Through this lab experiment we were able to see that diffusion did take a chance faster in the warm piss by seeing the fastest change in color of the table of contents inside the baggie. B. Did the sizes of the molecules affect their movement? Yes, the smaller the molecules (KI) were able to permeate thru the bag where as the bigger molecules (water) and corn whisky start could not permeate thru the membrane bag due to their large size. C.Give an example of diffusion between a solid and a liquid a gas and a liquid between dickens different solids. (solid/solid) Gold and lead- over time, molecules of lead bequeath diffuse into the gold and vice versa. (solid/liquid) dent and water- Sugar will dissolve in water. (Gas/liquid)Perfume and air- the scent will spread. Exercise 2 Osmosis Observations DataTable 2 Potato locoweed Contents in TestTube Initial Mass of Two Potato Strips Final Mass of Two Strips MassDifference % assortment inMass Class Average Percent Change in Mass (if available) a) Distilled pee 3. g 5g 1. 4 38% b) 1. 0 M saccharose 3. 4g 2. 1g -1. 3 -38% c) 0. 8 M Sucrose 3. 6g 2. 1g -1. 5 42% d) 0. 6 M Sucro se 3. 5g 2. 6g -. 9 -25% e) 0. 4 M Sucrose 3. 5g 3g -. 5 -14% f) 0. 2 M Sucrose 3. 4g 3. 7g . 3 9% Questions A. Describe how the tater cylinders changed in their turgidity. Identify which of the solutions were isotonic, hypertonic, and hypotonic in relation to the potato. a) Distilled Water No change in turgidity. Hypotonic solution. b) 1. 0 M Sucrose Very turgid. Hypertonic solution. ) 0. 8 M Sucrose Turgid. Hypertonic solution. d) 0. 6 M Sucrose Moderately turgid. Hypertonic solution. e) 0. 4 M Sucrose Slighty turgid. Hypertonic solution. f) 0. 2 M Sucrose No change in turgidity. Hypotonic solution. B. What would happen to a red blood electric cell placed in distilled water? Why? Distilled water is a hypotonic solution which means in contains no solutes. According to the text (p. 72), Cells placed in a hypotonic solution plump up rapidly as water rushes into them. This means that a red blood cell would eventually pop. C.Create a graph of your experimental data by plott ing the percent change in potato mass against sucrose hoagyity. Label the x-axis as Molarity of Sucrose, and the y-axis as % Change in Mass. D. Determine the molar concentration of the sucrose contained in the potato strips by using the graph created in question C. At the point where the molar concentration of the sucrose in the potato would be isotonic to the sucrose, there would be no net change in the potato mass. On the graph, this is located at the 0% change. persist a line from this place on the y-axis to intersect the line.From the line draw a line megabucks to the x-axis to find the molarity. Molar concentration of sucrose in potato = . 3 M Exercise 3 The Effect of Solvents on Membrane System Observations Data Table 3 Color Intensity from modify Beet Cells TestTube Solution Treatment ColorIntensity0 10 Condition of Beet (turgid, flaccid, etc. ) a 70% Isopropyl intoxicant 10 Very flacid b 35% Isopropyl alcoholic drink 7 bendable c 17. 5% Isopropyl alcohol 3 Less firm d Distilled Water 0 Very firm. Questions A. What is the reasoning behind the use of egg whites?How does this part of the experiment relate to membranes? Egg whites would provide a lipid layer mimicking the cell membrane layer of a cell. Also egg white are largely composed of water this would also mimic the cell membrane. B. What do the results of test tube e tell about the feeling of isopropyl alcohol on egg whites? The effects of the alcohol on the on the egg whites were that it made the unshapely as almost if they had been cooked. C. What do the results of test tube f tell about the effect of isopropyl alcohol on oil?How does this relate to membranes? The oil sinks in relation to the alcohol. This is related to membranes due to the hydrophilic and hydrophobic aspects. Lipids arrange so that the hydrophobic tail regions are isolated from the surrounding polar fluid, causing the to a greater extent hydrophilic head regions to associate with the intracellular (cytosolic) and ext racellular faces of the resulting bilayer. D. Based on the results from test tubes a by d, which of the solutions possessd the most damage on the membrane system? Explain your answer.The solution with the highest concentration of Isopropyl alcohol cause the most damage to the membrane because exposure to high concentration of organic solvents would disrupt membrane integrity. E. employ the same data collection method, develop a hypothesis and use the scientific method to innovation an experiment that would show the effect of temperature stress on membranes. Extreme temperatures, mainly -5? C and 70? C, will significantly damage the cell membrane while the temperatures closer to room temperature will cause solely slight damage, if any.